Follow aseptic procedures in the laboratory environment
Overview
Follow aseptic procedures in the laboratory environment in
accordance with approved procedures and practices.
You will be required to demonstrate that you can work to
the relevant standard operating procedures, legislation and
organisational policy. You will follow Good Laboratory
Practice (GLP) and/or Good Clinical Practice (GCP)/Good
Manufacturing Practice (GMP) in accordance with the
relevant workplace procedures.
The activity is likely to be undertaken by someone in a
science related work setting, including individuals working
in hospitals, scientific laboratories, schools and universities.
Performance criteria
You must be able to:
P1 ensure that your work is carried out in accordance with
standard operating procedures
P2 dress in the appropriate personal protection equipment
(PPE) required for the clean room or clean work area
environment, in accordance with the correct procedure
P3 carry out visual quality checks on your personal
protection equipment prior to entering the working
environment
P4 follow the correct procedures for entering and exiting the
clean room or clean work area
P5 follow aseptic techniques in the laboratory
P6 remove personal protection equipment on completion of
clean room or clean work area activities, and dispose/store
in line with the correct procedure
P7 communicate the required information about the work
done, to authorised people, in accordance with
departmental and organisational procedures
Knowledge and Understanding
You need to know and understand:
K1 the health and safety requirements of the area in which
you are carrying out the laboratory activities
K2 the implications of not taking account of legislation,
regulations, standards and guidelines when conducting
laboratory activities
K3 the principles of Good Laboratory Practice (GLP) and/or
Good Clinical practice (GCP)/Good Manufacturing Practice
(GMP) applied in the workplace
K4 the importance of wearing protective clothing, gloves
and eye protection when handling materials and the
equipment used to contain and process them
K5 the manufactured materials and batch process tracking
and records system
K6 the types of handling and sorting system, and the
procedures used for materials undergoing processing in the
laboratory facilities
K7 the importance of correct identification, and any unique
organisational or laboratory numbers
K8 the organisational requirements for maintaining the
security of the workplace
K9 the lines of communication and responsibilities in your
department, and their links with the rest of the organisation
K10 the limits of your own authority and to whom you
should report if you have problems that you cannot resolve
K11 the specific safety precautions to be taken when
working in a clean room or clean work area environment
K12 the correct fitting and use of clothing and personal
protective equipment that must be worn in a clean room or
clean work area
K13 hazards associated with working in a clean room or
clean work area, with laboratory equipment
K14 how to put on clean room clothing and footwear
correctly
K15 the procedures for entering and exiting the clean room
or clean work area, and the authority needed to do so
K16 the classification of the relevant clean room or clean
work area, and how this impacts upon you
K17 the industry standards/classifications for clean rooms
and clean work areas
K18 the company requirements for clothing and personal
protective equipment, and the reasons why such clothing
and equipment must be used
K19 the procedures and methods for maintaining issued
clothing and personal protective equipment
K20 how to apply procedures for dealing with damaged or
dirty clothing and personal protective equipment
K21 how to store clothing and personal protective
equipment correctly
K22 the laundering/cleaning/maintenance procedures
relating to the issued clothing and personal protective
equipment
K23 the aseptic techniques that are applied and used in the
laboratory
K24 how to dispose correctly of single-use personal
protective equipment
K25 the policy and procedures relating to personal items
Scope/range
- prior to entering clean room, carry out all of the following:
1.1 use the correct issue of job instructions and
specifications
1.2 follow risk assessment procedures and COSHH
regulations
1.3 ensure that you are appropriately dressed and
uncontaminated before entering the area
1.4 carry out your activities in line with organisational
procedures
1.5 store accurate records of your activities, in accordance
with appropriate procedures - satisfy all the following company clean room/clean work
area requirements:
2.1 use appropriate clothing/personal protective equipment
(PPE) (such as suits, gowns, coats, hoods, hats, caps,
helmets, other headwear, boots, overshoes, other forms of
footwear, safety goggles, visors, gloves)
2.2 comply with hazard protection (such as breathing
apparatus, gloves, apron/smock, other forms of PPE or
clothing required)
2.3 deal appropriately with damaged or dirty clothing/PPE
(such as reporting damage, replacement, safe removal and
cleaning or disposal, subjected to acid/hazardous
substance spills, damaged/dirty labelling)
2.4 store specified clothing/PPE correctly when not in use
2.5 ensure the proper cleaning/laundering/maintenance of
clothing/PPE
2.6 dispose of single-use clothing and equipment in the
correct location
2.7 report any hazards or breaches of protocol - use three of the following types of personal protective
equipment for clean room working:
3.1 body suit
3.2 gloves
3.3 air supply
3.4 face mask
3.5 respirator
3.6 other (please specify) - use personal protective equipment in one of the following
clean room environments:
4.1 health/disease screening
4.2 drug development
4.3 biochemical processing
4.4 agro-biotech research
4.5 biotechnology processing
4.6 other (please specify) - identify and follow protocol methods and procedures that
satisfy all of the following
5.1 the safety of people
5.2 containment/integrity of the clean room/work area
5.3 containment/integrity of the specimen/product
5.4 appropriate industry standards and protocols - record details of the work activity, and communicate the
details to the appropriate people, using:
6.1 verbal report plus one method from the following:
6.2 written or typed report
6.3 computer-based record
6.4 specific company documentation
6.5 electronic mail